Primary Antibodies

Monoclonal

Monoclonal antibodies derive from a single unique parent cell. These single-parent cells are from hybrid antibody-expressing cells (Hybridomas), typically made by fusing myeloma cells with spleen cells from a host that has been immunized with the target antigen. Compared to polyclonal antibodies, monoclonal antibodies tend to be highly specific, reacting with only one epitope on the target antigen and reducing non-specific cross-reactivity. This lower cross-reactivity also results in lower background signal, allowing for clear, easy-to-read results.

Polyclonal

Specific polyclonal antibodies are a heterogeneous mix of antibodies, procured from an immune response of multiple B-lymphocytes. The polyclonal immunoglobulins (IgG) are then purified from the host serum. Because polyclonal antibodies are made up of a mixture of antibodies, they tend to be more representative of a natural immune response to an antigen. Each polyclonal antibody recognizes a different epitope on the same antigen; however, by targeting multiple epitopes, there is a higher likelihood of target detection.

Rockland’s polyclonal antibodies can be used for a range of applications including, ELISA, Western blot, immunohistochemistry, chromatin immunoprecipitation (ChIP), or flow cytometry.

Recombinant Antibodies

Experience the superior reproducibility, specificity, and scalability of Rockland’s recombinant antibodies. Their clones are created by isolating DNA from B cells found in the blood of hyperimmunized animals, then selected using a display library to extract the antibodies with optimal characteristics. These antibodies are expressed in either CHO or E. coli and consistently exhibit high sensitivity and purity, making them perfect for large, long-term studies.

Their portfolio offers recombinant antibodies suitable for studying cancer, cell signaling, inflammation, and autoimmune diseases. Their antibodies that target common cancer markers, such as HER2 and CD20, mimic commercial immunotherapies and are well suited for furthering immuno-oncology research. Additionally, their anti-epitope tag antibodies are single domain (sdAb) allowing for better penetration during in-vivo and live-cell imaging.

Recombinant Antibodies for IVD and Therapeutics

The recombinant antibody market is quickly expanding as researchers and industry leaders look for monoclonal antibody alternatives. Consistency, reproducibility, and global manufacturing strategies are driving the interest in recombinant, animal-free options. These recombinant antibodies have inherent advantages and disadvantages.

Epitope-Tag Antibodies

Improve expression levels, solubility, folding, purification, and more with Rockland’s epitope tag antibodies that are carefully selected for high specificity and sensitivity. Their variety of conjugation alternatives, such as chemiluminescent and fluorescent labels, provide flexibility of method development when localizing gene products in various cell types.

> Enhanced sensitivity & specificity
> Compatible with most immunoassays
> Rigorous quality control testing for robust performance
> Tested for the detection of recombinant proteins expressed in prokaryotic and eukaryotic systems

Agarose and magnetic bead conjugates are effective reagents for protein enrichment and immunoprecipitation ascell types. These bead conjugates are also effective reagents for protein enrichment and immunoprecipitation assays.

RFP and GFP

Experience the versatility of Rockland’s Green Fluorescent Protein (GFP) and Red Fluorescent Protein (RFP) antibodies and proteins, produced using proven development methods by experienced scientists to provide highly specific and reliable antibodies for any application.

> Reliable GFP antibodies raised against full-length GFP protein, capable of recognizing native GFP and its variants
> Highly referenced, affinity-purified RFP antibodies raised in whole RFP protein of wild type, capable of recognizing all RFP variants
> Conjugated options to HRP, biotin, and fluorescent dyes

Isotype and Loading Controls

Ensure equal protein loading between samples or confirm the performance of other reagents in your assay with Rockland’s loading controls, which are vital for the interpretation and validity of immunoassays. Control proteins can be added to samples or occur in highly expressing cells. Thier control proteins and loading control antibodies have been validated in-house for trusted, repeatable results.

Loading Control Antibodies

Isotype controls are reagents added to immunoassays that allow researchers to evaluate non-specific binding and background signals. Isotype control antibodies are unique to tissue types that monitor non-specific primary antibodies binding to cell surface Fc receptors. These isotype control antibodies are most often needed in flow cytometry and immunohistochemistry assays.

Cancer

Facilitate your cancer research with highly-characterized cancer antibodies from Rockland. Their antibodies have been highly-validated to recognize relevant cancer targets for studying metastasis, apoptosis, autophagy, metabolism, and more. Many of their antibodies have been developed in partnership with renowned institutions, such as the MD Anderson Cancer Center, The Wistar Institute, National Cancer Institute, and Emory University, to combine basic and applied research principles for the development of better biomedical research reagents. Choose Rockland’s cancer antibodies for reproducible results that support innovative research and lead to significant advances in the fight against cancer.

Cell Biology

Advance your research with Rockland’s extensive collection of cell biology antibodies, encompassing core research areas, including signal transduction, cancer, neuronal research, developmental processes, cell structure, mitosis, DNA modification and others. Their reagents are tested in numerous applications to perform as specified, delivering highest quality tools for innovative research using microscopy, in vivo studies, cell-based assays, and flow cytometry.

Antibodies for Stem Cell Research

Stem cell antibodies facilitate stem cell research, which remains a growing area of scientific exploration, with numerous possibilities in various medical fields and disease treatments. Each differentiation state has specific markers that can be used to isolate or identify differentiated vs. non-differentiated cells.

Rockland produces antibodies against common pluripotent stem cell (PSCs) and multipotent stem cell markers, including Oct-4, GATA4, and Nanog antibodies.

Cell Signaling Pathways

Investigate a variety of cell signaling pathways with Rockland’s comprehensive portfolio of products, including primary antibodies, cytokines, growth factors, and cell viability assays.

Cell signaling or signal transduction allows cells to communicate and send signals from the cell surface to the nucleus. This interaction of cells requires many different proteins, such as growth and transcription factors, to activate membrane receptors inside the cell before a physiological effect on the cell’s behavior can be produced.

Any disruption or errors found within this communication chain gives rise to various diseases and cancers. So by understanding cell signaling, diseases may be treated more effectively and cures can be found.

COVID-19

The COVID-19 pandemic has challenged researchers worldwide, creating a common goal to understand the SARS-COV-2 virus, how to eradicate it, and how to prevent future coronavirus outbreaks. Rockland has a long-established history of providing critical antibodies and reagents as a qualified raw material supplier—specifically in the area of infectious disease and in vitro diagnostics.

Their antibodies specific to SARS-CoV-2 Nucleocapsid and Spike proteins are being used both directly and indirectly for vaccine development, drug discovery, diagnostics, and COVID-19 research by their global network of collaborators. Since the discovery of SARS-CoV-2, Rockland has been producing antibodies targeting various viral proteins, including the Membrane protein, 3CL Protease, and Non-Structural Protein 3 (NSP3) and 8 (NSP8).

Epigenetics

Rockland’s Epi-Plus™ line of highly specific, validated, antibodies recognize modified histones and related cellular targets. Epi-Plus™ antibodies are extremely sensitive and specific to the modification and show no cross-reactivity to other modifications on the same site or other amino acids of the protein. These antibodies are validated for immunofluorescence microscopy (IF), immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), immunohistochemistry (IHC), and Western blot (WB) analysis.

Rockland carries panels of chromatin and nuclear signaling antibodies containing the highest quality reagents available for epigenetics research. These epigenetic antibodies are produced against Histone PTMs under rigorous quality control testing.

Immunology

Enhance immune system and infectious disease research with Rockland’s extensive collection of primary antibodies that cover key areas of immunology, including cell surface markers, cytokine antibodies (including interferon gamma antibodies), T cell receptors, MHC molecules, and their protein partners.

We produce antibodies to specific diseases like Lyme disease, SARS, and influenza, including several monoclonal antibodies to H5N1 (also known as A (H5N1), a subtype of Influenza A. Their selection of antibodies also includes a diverse set of specific antibodies to modified proteins, such as phospho-specific and methylation-specific antibodies, Rockland also caters to the complement system, immunotherapy, autoimmune diseases, immuno-oncology.

If you’re interested in the detection of cell surface proteins using flow cytometry, we offer a wide range of fluorescence-conjugated, biotin-conjugated, and unconjugated primary antibodies with fluorescent dyes including traditional fluorescein (FITC), phycoerythrin (PE) and allophycocyanin (APC).

Neuroscience

Better identify and explain reactions on a cellular, molecular, and biochemical level with Rockland’s neuroscience antibodies. Antibody-based approaches for isolation, characterization, and localization of target proteins are among the most critical and widely used techniques in molecular and cellular neuroscience, leading to rapid development in their knowledge of protein abundance, distribution, structure, and function.

Rockland’s neuroscience antibodies exhibit high specificity, selectivity, and reproducibility by Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), and ELISA. These antibodies are routinely used for characterization of neural development, stem cell and differentiation, neural transmission, neurodegenerative disease and neuroinflammation.