Protein G Agarose & Magnetic Beads

Simply add the ChIP reaction to the columns and use the cap to seal. The incubation and wash steps are performed within the column to streamline the process and prevent any loss of material. The columns offer a faster solution to centrifugation and magnetic separation methods and result in better reproducibility between samples because no material is lost.

Protein G Agarose Beads
Alternatively, Protein G Agarose Beads are available without the columns. The beads have a binding capacity of 10 µg IgG/µl bead and are provided as a 50% slurry. Beads should be washed with TE, pH 8.0 prior to use in chromatin immunoprecipitation reactions.

ChIP-IT Fixation Buffer
Additionally, Active Motif has the ChIP-IT® Fixation Buffer available as a stand alone reagent. This specially formulated buffer can be used during the formaldehyde fixation step of chromatin preparation to help eliminate pH effects during chromatin preparation and provide better consistency among chromatin preparations, resulting in better ChIP results. Simply add formaldehyde to the ChIP-IT Fixation Buffer and add directly to the cell culture growth medium. Then proceed with the ChIP protocol of your choice.

Protein G Magnetic Beads
For researchers looking to use magnetic beads, Active Motif offers the ChIP-IT® Protein G Magnetic Beads. These are the same Protein G magnetic beads included in the ChIP-IT® Express Kits.

Brand: Active Motif
Categories: Antibodies, Media, Kits and Other Reagents, Assay and Extraction Kits